was used to determine concentrations of lactate (Abcam, Abcam, ab13847), anti-mitochondrial fission protein 1 (Fis1; 1:1000, Abcam, ab71498), anti-mitofusin

Pro Caspase 3, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

The following day, after being washed with PBS buffer, the sections were incubated first with secondary antibodies for 1 hour at room temperature and then with horseradish peroxidase (HRP)-coupled streptavidin. アブカムは、抗体、elisaキットなど10万を超える試薬製品の提供、使いやすい製品検索、豊富な在庫と迅速な配送、最新技術情報の発信などを通じ、ライフサイエンス研究をサポートします。 The near-infrared dye IR-61 restores erectile function in a streptozotocin-induced diabetes model via mitochondrial protection Xiao-Feng Yue 1, Chong-Xing Shen 1, Jian-Wu Wang 1, Lin-Yong Dai 1, Qiang Fang 1, Lei Long 2, Yi Zhi 1, Xue-Ru Li 3, Ya-Wei Wang 2, Gu-Fang Shen 2, Zu-Juan Liu 2, Chun-Meng Shi 2, Wei-Bing Li 1 The detection was made using ECL detection kit (Abcam, ab65623, MA, USA) with 5 min incubation in a dark room. The detection reagent was drained and then, the membrane was exposed to a sheet of diagnostic film in a film cassette for 1 to 30 min . active Caspase 3 human, reacts with human, mouse, rat, fruit fly (drosophila melanogaster), indian muntjac, rhesus monkey, xenopus laevis and zebrafish (pmid 19823566)predicted to react with chinese hamster (100 identity with immunogen), dog (100 identity with immunogen) and pig (100 identity with immunogen) due to sequence homology 6044 | CHE E T AL. up-regulation of several osteogenic genes, including runt-related tran-scription factor 2 (Runx2), alkaline phosphatase (ALP) and osteocalcin.5 caspase3 (ab49822, Abcam), total caspase3 (ab13847, Abcam), E-cadherin (CST, Danvers, MA, USA) and β-actin (cat. no. 8457, Cell Signaling Technology) were added respectively for overnight incubation at 4°C. Sec-ondary antibodies including goat anti-rabbit (ab205718, Abcam) and goat anti-mouse (ab6789, Abcam) were 2020-01-30 · The membranes were blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBST) for 2 h at room temperature, and then incubated overnight at 4 °C with primary antibodies (Abcam, Shanghai China) against B-cell lymphoma-2 (Bcl-2, ab32124), Bcl-2-associated X (Bax, ab32503), total poly (ADP-ribose) polymerase (PARP, ab74290), X-linked inhibitor of apoptosis (XIAP, ab21278 2018-04-03 · Slides were placed on the Bond Max autostainer for staining with the Abcam antibody for Active Caspase 3 (Rabbit polyclonal, ab13847).

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UniProt Code History. A8K5M2, D3DP53, P42574, Q96AN1 ab13847 was shown to recognise Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE. ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C.

Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of Caspase-3 Antibody (31A1067) - (Pro and Active). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST.

anti-Caspase-3. (Abcam,ab13847), anti-Bcl-2. (Abcam,ab196495), anti-Bax ( Abcam,ab53154) ve TUNEL (In Situ Cell Death Detection Kit,. POD;. 11684817910.

ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells. Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application.

10 Feb 2021 (PCNA, AB13847, Abcam, MA) or an IgG that recognizes cleaved caspase-3 (1: 250, AB13847, Abcam, MA), respectively. Rat anti- mouse CD3 

If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team Citations for ab13847 by Abcam. 781 publications reference this Antibody.

Rabbit anti- mouse CD31 ab28364. Abcam.
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Scientific. Rabbit anti-mouse Ki-67 ab15580. Abcam.

2020-09-25 · And then, slices were incubated with GRP78 (Abcam, ab21685, rabbit, 1: 2000) overnight at 4 °C. After incubation, slices were washed by PBS and incubated with FITC-labeled IgG (Abcam, ab74290, 1:2000) for 45 min at room temperature.
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and -BAX (Ab3154) were purchased from Abcam (Cambridge, UK). An anti-active caspase-3 specific antibody was purchased from Abcam (Ab13847) to detect the cleaved 17 kD form of caspase-3 by Western blotting. Protein AG agarose was purchased from Pierce (Waltham, MA, USA). HEK293 (RRID:CVCL_0045) and 769-P (RRID:CVCL_1050) kidney

Abcam catalog: ab13847 total number of products: 19. domestic rabbit polyclonal reactivity: human, mouse, rat, Xenopus laevis application: western blot 产品报价：询价 更新时间：2013/6/20 13:23:01 产地：美国. 品牌：Abcam. 型号：ab13847. 厂商性质： 生产型,贸易型,服务型, domestic rabbit monoclonal (E61) reactivity: human conjugate: HRP application: western blot, immunohistochemistry - paraffin section Hu Y, Guo F, Xu Y, Li P, Lu Z, McVey D, et al.Long noncoding RNA NEXN-AS1 mitigates atherosclerosis by regulating the actin-binding protein NEXN. J Clin Invest.